Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation （ヒト線維柱帯細胞への水流機械刺激による細胞外マトリックス遺伝子発現に関する研究）
著者
吉田, 光一
(Yoshida, Koichi)
上位タイトル
International journal of ophthalmology
Vol.15,
No.3
(2022.
3)
,p.388-
393
識別番号
ISSN
2222-3959
DOI
10.18240/ijo.2022.03.03
その他
PMID:35310043
博士論文情報
学位授与番号
10107B494
学位授与年月日
2022-06-30
学位名
博士（医学）
学位授与機関
旭川医科大学
抄録
Aim: To investigate changes in extracellular matrix (ECM) gene expression in human trabecular meshwork (HTM) cells in response to mechanical fluid flow stimulation.
Methods: HTM cells were grown on a glass plate coated with 0.02% type I collagen (COL) and exposed to shear stress (0, 0.2, 1.0 dyne/cm2) for 12h. Changes in genes related to the ECM were evaluated by real-time reverse transcriptase-polymerase chain reaction. Phosphorylation of Smad2 protein was investigated by Western blotting.
Results: After mechanical stimulation, COL type 4 alpha 2, COL type 6 alpha 1, and fibronectin-1 mRNA were significantly higher than the static control (P<0.05, <0.05, and <0.01, respectively). The metalloproteinase-2 and plasminogen activator inhibitor-1 mRNA were significantly higher than the static control (P<0.05 and <0.01, respectively), while the differences in the tissue inhibitors of metalloproteinases-2 mRNA were not significant. The phosphorylation of Smad2 levels was significantly higher compared to the static control cells.
Conclusion: Changes in the expressions of genes associated ECM metabolism result in HTM cells after mechanical stimulation. The mechanical stimulation of the aqueous humor to the trabecular meshwork may promote ECM turnover and contribute to intraocular pressure homeostasis
