Capillary-resident EphA7+ pericytes are multipotent cells with anti-ischemic effects through capillary formation （EphA7陽性毛細血管周細胞は多分化能を有し、血管形成能を介して、組織虚血を改善する）
著者
吉田, 有里
(Yoshida, Yuri)
上位タイトル
Stem cells translational medicine
Vol.9,
No.1
(2020.
1)
,p.120-
130
識別番号
ISSN
2157-6564
DOI
10.1002/sctm.19-0148
その他
PMID: 31471947
博士論文情報
学位授与番号
10107A546
学位授与年月日
2020-03-25
学位名
博士（医学）
学位授与機関
旭川医科大学
抄録
The presence of pericytes (PCs) with multipotency and broad distribution along capillary suggests that microvasculature plays a role not only as a duct for blood fluid transport but also as a stem cell niche that contributes to tissue maintenance and regeneration. The lack of an appropriate marker for multipotent PCs still limits our understanding of their pathophysiological roles. We identified the novel marker EphA7 to detect multipotent PCs using microarray analysis of an immortalized PC library. PCs were isolated from microvessels of mouse subcutaneous adipose tissues, then EphA7+ PCs called capillary stem cells (CapSCs) were separated from EphA7- control PCs (ctPCs) using fluorescence-activated cell sorting system. CapSCs had highly multipotency that enabled them to differentiate into mesenchymal and neuronal lineages compared with ctPCs. CapSCs also differentiated into endothelial cells and PCs to form capillary-like structures by themselves. Transplantation of CapSCs into ischemic tissues significantly improved blood flow recovery in hind limb ischemia mouse model due to vascular formation compared with that of ctPCs and adipose stromal cells. These data demonstrate that EphA7 identifies a subpopulation of multipotent PCs that have high angiogenesis and regenerative potency and are an attractive target for regenerative therapies.
