Longitudinal Changes in Retinal Blood Flow in a Feline Retinal Vein Occlusion Model as Measured by Doppler Optical Coherence Tomography and Optical Coherence Tomography Angiography （ドップラ光干渉断層計と光干渉断層血管撮影を用いて測定したネコ網膜静脈閉塞モデルにおける網膜血流の継時的変化）
著者
和田, 剛成
(Wada, Takanari)
上位タイトル
Investigative ophthalmology and visual science
Vol.61,
No.2
(2020.
2)
,p.34-
識別番号
ISSN
0146-0404
DOI
10.1167/iovs.61.2.34
その他
PMID:32084269
博士論文情報
学位授与番号
10107A553
学位授与年月日
2020-12-25
学位名
博士（医学）
学位授与機関
旭川医科大学
抄録
Purpose
We aimed to observe longitudinal changes in retinal blood flow (RBF) and structural transformations in capillaries using Doppler optical coherence tomography (DOCT) and optical coherence tomography angiography (OCTA) in a feline retinal blood occlusion (RVO) model.
Methods
RVO was induced by argon green laser photocoagulation (PC) in six eyes of six cats. RBF was measured at a first-order retinal artery and vein by a DOCT flowmeter, and structural changes in the capillaries around the occluded vessels (12 × 12 and 3 × 3 mm) were assessed by OCTA before (at baseline); immediately after PC; and on days 1, 4, 7, and 14 thereafter. Systemic and ocular parameters were monitored during the observation period.
Results
There were no significant differences in any systemic or ocular parameters before and after PC. Arterial RBF increased significantly on day 1 (160.6 ± 8.6% vs. baseline, P < 0.001) and decreased below baseline after day 1 through 14. Venous RBF decreased immediately after PC (17.4 ± 9.6% vs. baseline, P = 0.001) and then gradually increased afterwards, but did not return to baseline. OCTA showed dilatation of retinal venules immediately after PC to day 1. Collateral vessels began to form on day 4, had matured by day 7, and were pruned on day 14, which formed as mature as normal retinal venule diameters.
Conclusions
With increasing arterial RBF within 1 day after inducing RVO, venules gradually expanded to form collateral vessels, suggesting that collateral vessels originate from existing capillary networks, not neovascularization.
