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閲覧数:882
ID 20180629_O473
アイテムタイプ Article
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本文 O473 Goto Takuma_TD.pdf
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タイトル An elevated expression of serum exosomal microRNA-191, - 21, -451a of pancreatic neoplasm is considered to be efficient diagnostic marker.
別タイトル
血清エクソソーム中microRNA-191,-21,-451aは膵腫瘍の有用な診断マーカーとなり得る
著者
後藤, 拓磨 (Goto, Takuma)
上位タイトル
BMC Cancer Vol.18, No.1  (2018. 1) ,p.116-
識別番号
ISSN
1471-2407
DOI 10.1186/s12885-018-4006-5.
その他
PMID:29385987
博士論文情報
学位授与番号 10107B473
学位授与年月日 2018-6-29
学位名 博士(医学)
学位授与機関 旭川医科大学
抄録 BACKGROUND:

Pancreatic cancer is associated with an extremely poor prognosis, so new biomarkers that can detect the initial stages are urgently needed. The significance of serum microRNA (miR) levels in pancreatic neoplasm such as pancreatic cancer and intraductal papillary mucinous neoplasm (IPMN) diagnosis remains unclear. We herein evaluated the usefulness of miRs enclosed in serum exosomes (ExmiRs) as diagnostic markers.
METHODS:

The ExmiRs from patients with pancreatic cancer (n = 32) or IPMN (n = 29), and patients without neoplasms (controls; n = 22) were enriched using ExoQuick-TC™. The expression of ExmiRs was evaluated using a next-generation sequencing analysis, and the selected three miRs through this analysis were confirmed by a quantitative real-time polymerase chain reaction.
RESULTS:

The expression of ExmiR-191, ExmiR-21 and ExmiR-451a was significantly up-regulated in patients with pancreatic cancer and IPMN compared to the controls (p < 0.05). A receiver operating characteristic curve analysis showed that the area under the curve and the diagnostic accuracy of ExmiRs were 5-20% superior to those of three serum bulky circulating miRs (e.g.; ExmiR-21: AUC 0.826, accuracy 80.8%. Circulating miR-21: AUC 0.653, accuracy 62.3%). In addition, high ExmiR-451a was associated with mural nodules in IPMN (p = 0.010), and high ExmiR-21 was identified as a candidate prognostic factor for the overall survival (p = 0.011, HR 4.071, median OS of high-ExmiR-21: 344 days, median OS of low-ExmiR-21: 846 days) and chemo-resistant markers (p = 0.022).
CONCLUSIONS:

The level of three ExmiRs can thus serve as early diagnostic and progression markers of pancreatic cancer and IPMN, and considered more useful markers than the circulating miRs (limited to these three miRs).
キーワード
Exosome
Intraductal papillary mucinous neoplasm
Pancreatic cancer
Tumor marker
microRNA-21
microRNA-451a
言語
eng
資源タイプ application/pdf
ジャンル Thesis or Dissertation
著者版フラグ ETD
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