AMCoR Asahikawa Medical College
HOME
|

AMCoR:Asahikawa Medical University Collection and Research (旭川医科大学学術成果リポジトリ)は、本学で生産された電子的な知的生産物(学術雑誌論文の原稿・教材・学術資料など)を保存し、原則的に無償で発信するためのインターネット上の保管庫です。

※AMCoRに収録された学術論文のほとんどは、商業出版社や学会出版社の学術雑誌に掲載されたものですが、著作権に係わる出版社の方針により、出版社の条件に添った版を収録しています。そのため実際の誌面とはレイアウトの相違や、字句校正による文言の違いがあり得ますことをあらかじめご了承ください。


| ホーム ニュース ログイン |

Language

AMCoR検索
  
     詳細検索

インデックスツリー

詳細



閲覧数:4150
ID 11821288
アイテムタイプ Article
このアイテムを表示する
本文 321.pdf
Type : application/pdf Download
Size : 179.3 KB
Last updated : Dec 4, 2007
Downloads : 1078

Total downloads since Dec 3, 2007 : 1078
タイトル A cytogenetic study of in-vitro matured murine oocytes after ICSI by human sperm
著者
千石, 一雄 (Sengoku, Kazuo)
Tsuchiya, K
Kamiguchi, Y
Ishikawa, M
上位タイトル
Human Reproduction Vol.17, No.2  (2002. 2) ,p.420- 425
識別番号
ISSN
0268-1161
DOI 10.1093/humrep/17.2.420
URI http://humrep.oxfordjournals.org/cgi/reprint/17/2/420
抄録 BACKGROUND: The purpose of this study was to investigate the chromosomal complement and developmental potential of in-vitro matured murine oocytes following ICSI by human sperm. METHODS: Heterologous ICSI fertilization between mouse oocytes and human sperm was employed in order to overcome the reduced fertilization rates observed after conventional IVF due to zona hardening during in-vitro maturation, and to assess separately maternal and paternal chromosome complements. Cytogenetic analyses were performed in four types of oocytes: (I) in-vitro matured metaphase II (MII) oocytes; (ii) in-vivo matured MII oocytes; (iii) in-vitro matured oocytes after ICSI; (iv) in-vivo matured oocytes after ICSI. RESULTS: Activation rates after ICSI of in-vitro matured oocytes was lower than that of in-vivo matured oocytes (69.9 versus 97.2%, P < 0.01), and premature chromosomal condensation was only observed in in-vitro matured oocytes. However, there were no significant differences in developmental rates after successful activation between in-vivo and in-vitro matured ICSI oocytes (69.7 versus 76.6%). The incidences of aneuploidy and structural aberrations were similar between the ICSI embryos and non-ICSI (MII) oocytes. Furthermore, the frequency of chromosomal aberrations was not associated with in-vitro or in-vivo maturation. Similar analyses of paternal chromosomes indicated that there were no significant differences in the incidence of chromosomal aberrations between the embryos derived from in-vitro and in-vivo matured oocytes. CONCLUSIONS: These results suggest that in-vitro matured oocytes following ICSI do not lead to an increase in the frequency of aneuploidy and structural aberrations when human sperm are injected into mouse oocytes.
注記 This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Oxford University Press, Human Reproduction, 17(2), 2002, 420-425 is available online at: http://humrep.oxfordjournals.org/cgi/content/abstract/17/2/420.

author
言語
eng
資源タイプ text
ジャンル Journal Article
Index
/ Public
/ Public / 国外雑誌論文
関連アイテム