Effects of chronic treatment with cilostazol, a phosphodiesterase 3 inhibitor, on female rat bladder in a partial bladder outlet obstruction model.
著者
松本, 成史
(Matsumoto, Seiji)
渡邊, 成樹
(Watanabe, Masaki)
橋爪, 和純
(Hashizume, Kazumi)
和田, 直樹
(Wada, Naoki)
堀, 淳一
(Hori, Junichi)
北, 雅史
(Kita, Masafumi)
岩田, 達也
(Iwata, Tatsuya)
柿崎, 秀宏
(Kakizaki, Hidehiro)
上位タイトル
Urology
Vol.83,
No.3
(2014.
3)
,p.675.e7-
675.e11
識別番号
ISSN
0090-4295
DOI
10.1016/j.urology.2013.11.030.
その他
PMID:24581543
抄録
OBJECTIVE:
To investigate whether bladder dysfunction after bladder outlet obstruction (BOO) could be altered by treatment with cilostazol, a phosphodiesterase 3 inhibitor (PDE3i).
METHODS:
Twelve-week-old female Sprague-Dawley rats were divided into 5 groups: groups 1 and 2, sham-operated rats and groups 3-5, BOO rats. Group 1 and 3 rats were given normal diet, group 2 and 5 rats were given high-dose PDE3i diet, and group 4 rats were given low-dose PDE3i diet. PDE3i was given within diet from the day of surgery. Four weeks after BOO, the bladder was excised and dissected into 4 longitudinal strips for isometric organ-bath assay. Contractile responses of bladder strips to electrical field stimulation (EFS), carbachol, and potassium chloride (KCl) were determined for each group.
RESULTS:
BOO induced a significant increase in bladder weight in groups 3-5 compared with groups 1 and 2. PDE3i treatment did not affect bladder weight in sham or BOO rats. Contractile forces in response to EFS, carbachol, and KCl in group 3 were about 20%-40% of those in group 1. Contractile responses to EFS or KCl in PDE3i-treated BOO rats were not significantly different from those in group 3. Only high dose of PDE3i treatment in BOO rats caused a statistically significant increase in the response to carbachol compared with group 3.
CONCLUSION:
PDE3i has a small but significant protective effect on the contractile dysfunction induced by a 4-week BOO in rats, although the increase in bladder mass was not altered. PDE3i could be a useful protection against contractile dysfunction of the obstructed bladder.
