T-cell activation in tonsils of patients with pustulosis palmaris et plantaris
著者
高原, 幹
(Takahara, Miki)
Kishibe, K
Nozawa, H
Harabuchi, Y
上位タイトル
International Congress Series
Vol.1257,
(2003.
12)
,p.163-
166
識別番号
ISSN
0531-5131
DOI
10.1016/S0531-5131(03)01618-2
抄録
Pustulosis palmaris et plantaris (PPP) is a well-known skin disease closely related to tonsillar focal infections, and tonsillectomy is very effective for treatment of this condition. However, etiology of PPP was unclear. In this study, we investigated the characteristics of tonsils from PPP patients by clinical, immunohistochemical, immunological, and molecular–biological approach, and considered the etiology of PPP. For 47 Japanese patients with PPP who have tonsillectomy in Asahikawa Medical College, the skin lesion of PPP was improved in 87% of PPP patients 12 months after tonsillectomy. In quantitative immunohistologic analysis by measurement of T-cell nodule areas on tonsillar sections from those patients, there was positive correlation between the enlargement and improvement of the skin lesion. These results suggested that the T-cell nodule expansion might be an important clue towards clarifying the pathogenesis of this tonsil related disease. In flow cytometric analysis, CD3+, CD4+ and CD4+CD25+ cells were significantly elevated in tonsillar lymphocyte from PPP patients. In reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting analyses of TGF-beta, IL-10, and Smad group, Smad7 mRNA and protein in tonsillar CD3+ cells from PPP patients were expressed at higher level than those from obstructive sleep apnea syndrome (OSAS) patients. These results suggested that helper T cells may be frequently activated and proliferating in tonsils of PPP patients, and the activation and proliferation of helper T cells may be due to enhanced inhibition by Smad7 in intracellular signal-transduction of TGF-beta.
