Comparison of the serological tests ICT and ELISA for the diagnosis of alveolar echinococcosis in France.
著者
Jenny, Knapp
迫, 康仁
(Sako, Yasuhito)
Frédéric, Grenouillet
Solange, Bresson-Hadni
Carine, Richou
Houssein, Gbaguidi-Haore
伊藤, 亮
(Ito, Akira)
Laurence, Millon
上位タイトル
Parasite
No.21
(2014.
7)
,p.1-
6
識別番号
ISSN
1252-607X
DOI
10.1051/parasite/2014037.
その他
PMID：25058754
抄録
Serological diagnosis of alveolar echinococcosis (AE) is a key element for efficient patient treatment management. A rapid immunochromatography test kit (ICT) using the recombinant Em18 antigen (rEm18) was recently developed. The aim of our study was to assess this test on a panel of sera from French patients with alveolar echinococcosis and control patients. In a blind test, a total of 112 serum samples were tested including samples of AE (n = 30), cystic echinococcosis [CE] (n = 15), and polycystic echinococcosis [PE] (n = 1). For the comparison, 66 sera from patients with hepatocarcinoma, fascioliasis, toxocariasis, Caroli's disease, or autoimmune chronic active hepatitis were used. The diagnostic test sets we used were the rEm18-ICT and two validated ELISAs with rEm18 and Em2-Em18 antigens, respectively. For the ICT, 27/30 sera from AE patients, 4/15 sera from CE patients and the PE patient serum were positive. One serum from the control panel (toxocariasis) was positive for the ICT. The rEm18-ICT sensitivity (90.0%) and specificity (92.7%) for detection of Em18-specific antibodies confirmed it as a relevant tool for AE diagnosis. The rEm18-ELISA had a sensitivity of 86.7% and specificity of 91.5%, and the Em2-Em18-ELISA had a sensitivity of 96.7% and specificity of 87.8%. However, when AE patient sera are recorded as weak in intensity with the ICT, we recommend a double reading and use of a reference sample if the ICT is used for patient follow-up.
キーワード
alveolar echinococcosis
diagnosis
rEm18
immunochromatography
rapid test
rEm18-ELISA and Em2-Em18-ELISA tests
注記
This is an Open Access article distributed under the terms of the Creative Commons Attribution License
