Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis.
著者
迫, 康仁
(Sako, Yasuhito)
Ito, Sonoyo
Okamoto, Munehiro
Nakaya, Kazuhiro
Ito, Akira
上位タイトル
Parasitology
Vol.21,
(2013.
1)
,p.1-
6
識別番号
ISSN
0031-1820
DOI
10.1017/S0031182013000978
その他
PMID:23790268
抄録
SUMMARY Cysticercosis caused by infection with the larval stage of Taenia solium is an important cause of neurological disease worldwide and immunodiagnosis is important for the control and elimination of cysticercosis. In the present study, we established a simple and reliable preparation of immunodiagnostic low-molecular-weight antigens (LMWAgs) from T. solium cyst fluids by a cation-exchange chromatography (CEC). Banding patterns of LMWAgs on SDS-PAGE were different between isolates from Ecuador and China. All cysticercosis patient sera and some echinococcosis patient sera recognized both LMWAgs by enzyme-linked immunosorbent assay (ELISA), but sera from healthy persons were not positive. There was no statistical difference in immunodiagnostic performance of LMWAgs prepared from different geographical isolates. These results indicated that these novel immunodiagnostic antigen preparations could contribute the control and prevention of cysticercosis in endemic areas, especially developing countries.
