Water-avoidance stress enhances gastric contractions in freely moving conscious rats: role of peripheral CRF receptors
著者
野津, 司
(Nozu, Tsukasa)
Kumei, Shima
Takakusaki, Kaoru
Okumura, Toshikatsu
上位タイトル
Journal of Gastroenterology
Vol.49,
No.5
(2014.
5)
,p.799-
805
識別番号
ISSN
0944-1174
DOI
https://doi.org/10.1007/s00535-013-0828-8
その他
PMID:23645119
抄録
Background. Stress alters gastrointestinal motility through central and peripheral corticotropin-releasing factor (CRF) pathways. Accumulating evidence has demonstrated that peripheral CRF is deeply involved in the regulation of gastric motility, and enhances gastric contractions through CRF receptor type 1 (CRF1) and delays gastric emptying (GE) through CRF receptor type 2 (CRF2). Since little is known whether water-avoidance stress (WAS) alters gastric motility, the present study tried to clarify this question and the involvement of peripheral CRF receptor subtypes in the mechanisms. Methods. We recorded intraluminal gastric pressure waves using a perfused manometric method. The rats were anesthetized and the manometric catheter was inserted into the stomach 4−6 days before the experiments. We assessed the area under the manometric trace as the motor index (MI), and compared this result with those obtained 1 h before and after initiation of WAS in non-fasted conscious rats. Solid GE for 1 h was also measured. Results. WAS significantly increased gastric contractions. Intraperitoneal (ip) administration of astressin (100 g/kg, 5 min prior to stress), a non-selective CRF antagonist, blocked the response to WAS. On the other hand, pretreatment (5 min prior to stress) with neither astressin2-B (200 g/kg, ip), a selective CRF2 antagonist, nor urocortin 2 (30 g/kg, ip), a selective CRF2 agonist, modified the response to WAS. These drugs did not alter the basal MI. WAS did not change GE. Conclusions. WAS may activate peripheral CRF1 but not CRF2 signaling and stimulates gastric contractions without altering GE.
