Suppression of lipin-1 expression increases monocyte chemoattractant protein-1 expression in 3T3-L1 adipocytes
著者
高橋, 伸彦
(Takahashi, Nobuhiko)
Yoshizaki, Takayuki
Hiranaka, Natsumi
Suzuki, Takeshi
Yui, Tomoo
Akanuma, Masayasu
Oka, Kazuya
Kanazawa, Kaoru
Yoshida, Mika
Naito, Sumiyoshi
Fujiya, Mikihiro
Kohgo, Yutaka
Ieko, Masahiro
上位タイトル
Biochemical and Biophysical Research Communications
Vol.415,
No.1
(2011.
11)
,p.200-
205
識別番号
ISSN
0006-291X
DOI
10.1016/j.bbrc.2011.10.060
抄録
Lipin-1 plays a crucial role in the regulation of lipid metabolism and cell differentiation in adipocytes. Expression of adipose lipin-1 is reduced in obesity, and metabolic syndrome. However, the significance of this reduction remains unclear. This study investigated if and how reduced lipin-1 expression affected metabolism. We assessed mRNA expression levels of various genes related to adipocyte metabolism in lipin-1-depleted 3T3-L1 adipocytes by introducing its specific small interfering RNA. In lipin-1-depleted adipocytes, mRNA and protein expression levels of monocyte chemoattractant protein-1 (MCP-1) were significantly increased, although the other genes tested were not altered. The conditioned media from the cells promoted monocyte chemotaxis. The increase in MCP-1 expression was prevented by treatment with quinazoline or salicylate, inhibitors of nuclear factor-κB activation. Because MCP-1 is related to adipose inflammation and systemic insulin resistance, these results suggest that a reduction in adipose lipin-1 in obesity may exacerbate adipose inflammation and metabolism.
