Molecular cloning and functional analysis of scavenger receptor zebrafish CL-P1
著者
福田, 光子
(Fukuda, Mitsuko)
大谷, 克城
(Ohtani, Katsuki)
Jang, Seong-Jae
吉崎, 隆之
(Yoshizaki, Takayuki)
森, 健一郎
(Mori, Ken-ichiro)
本村, 亘
(Motomura, Wataru)
吉田, 逸朗
(Yoshida, Itsuro)
鈴木, 定彦
(Suzuki, Yasuhiko)
高後, 裕
(Kohgo, Yutaka)
若宮, 伸隆
(Wakamiya, Nobutaka)
上位タイトル
Biochimica et Biophysica Acta (BBA) - General Subjects
Vol.10,
No.12
(2011.
12)
,p.1150-
1159
識別番号
ISSN
0006-3002
その他
PMID:.22001438
抄録
BACKGROUND:
Scavenger receptors are generally expressed in macrophages and vascular endothelial cells and some scavenger receptors are thought to contribute to the development of atherosclerosis.
METHODS:
We cloned the cDNA of a zebrafish CL-P1 (collectin placenta 1) and performed a knockdown study using its antisense morpholino oligonucleotides (MO).
RESULTS:
Zebrafish CL-P1 (zCL-P1) is 51% identical to human CL-P1 in its amino acid sequence. Microbes and OxLDL bound to zCL-P1 cDNA transfected cells. zCL-P1 mRNA expression gradually increased after 6hours post-fertilization (hpf), reached its highest level at 24hpf, and then decreased, which is similar to the gene expression pattern of Tie-2. The knockdown of zCL-P1 led to an increase in the number of zebrafish embryos with severe morphological abnormalities such as short body lengths and defects in the dorsal aorta at 48hpf. Simultaneous injection of both MO and synthetic zCL-P1 or zVEGF mRNA rescued the abnormal phenotype.
CONCLUSIONS:
In vivo knockdown study shows that zCL-P1 is implicated in vasculogenesis and those of our in vitro study support its role as a scavenger receptor.
GENERAL SIGNIFICANCE:
These results suggest that zCL-P1 might be essential for vasculogenesis during the early embryonic phase in bone fish.
