Fluorescent and colored trinitrophenylated analogs of ATP and GTP
著者
平塚, 寿章
(Hiratsuka, Toshiaki)
上位タイトル
European Journal of Biochemistry
Vol.270,
No.17
(2003.
9)
,p.3479-
3485
識別番号
ISSN
0014-2956
DOI
10.1046/j.1432-1033.2003.03748.x
抄録
Fluorescent and colored trinitrophenylated (TNP) analogs of ATP and GTP can interact with nucleotide-requiring enzymes and proteins as a substitute for the parent nucleotide. These analogs have strong binding affinities for most nucleotide-requiring systems. Their bindings are easily detected by absorption and fluorescence changes in the visible region. Recent years have seen dramatic developments in the application of the TNP nucleotide analogs as spectroscopic probes for the study on the nucleotide-interacting properties of various enzymes and proteins including their mutants. This review is intended as a broad overview of currently extensively used applications of the nucleotide analogs in various biological systems.
