Identification and Characterization of a Novel Human Collectin CL-K1
著者
芥子, 宏行
(Keshi, Hiroyuki)
Sakamoto, Takashi
河合, 高生
(Kawai, Takao)
加藤, 剛志
(Katoh, Tsuyoshi)
Jang, Seong-Jae
本村, 亘
(Motomura, Wataru)
吉崎, 隆之
(Yoshizaki, Takayuki)
福田, 光子
(Fukuda, Mitsuko)
小山, 聡
(Koyama, Satoshi)
福澤, 純
(Fukuzawa, Jun)
福應, 温
(Fukuoh, Atsushi)
吉田, 逸朗
(Yoshida, Itsuro)
鈴木, 定彦
(Suzuki, Yasuhiko)
若宮, 伸隆
(Wakamiya, Nobutaka)
上位タイトル
Microbiology and Immunology
Vol.50,
No.12
(2006.
12)
,p.1001-
1013
識別番号
ISSN
0385-5600
DOI
10.1111/j.1348-0421.2006.tb03868.x
その他
PMID:17179669
抄録
Collectins are a family of C-type lectins with two characteristic structures, collagen like domains and carbohydrate recognition domains. They recognize carbohydrate antigens on microorganisms and act as host-defense. Here we report the cloning and characterization of a novel collectin CL-K1. RT-PCR analyses showed CL-K1 mRNA is present in all organs. The deduced amino acid sequence and the data from immunostaining of CL-K1 cDNA expressing CHO cells revealed that CL-K1 is expressed as a secreted protein. CL-K1 is found in blood by immunoblotting and partial amino acid analyses. CL-K1 showed Ca(2+)-dependent sugar binding activity of fucose and weakly mannose but not N-acetyl-galactosamine, N-acetyl-glucosamine, or maltose, though mannose-binding lectin (MBL) containing similar amino acid motif. CL-K1 can recognize specially several bacterial saccharides due to specific sugar-binding character. Elucidation of the role of two ancestor collectins of CL-K1 and CL-L1 could lead to see the biological function of collectin family.
