Albumin-producing hepatocytes derived from cryopreserved F344 rat bone marrow cells transplanted in the livers of congenic Nagase's analbuminemic rats.
著者
稲垣, 光裕
(Inagaki, Mitsuhiro)
Arikura, J
Huiling, X
Yan, X
Ogawa, K
Kasai, S
上位タイトル
Surgery Today
Vol.35,
No.11
(2005.
11)
,p.955-
960
Purpose. Hematopoietic stem cells (SC) are though to have the potential to differentiate into hepatocytes; however, this potential has not been reported for cryopreserved SCs. We investigated if cryopreserved bone marrow cells (BMCs) from F344 rats (F344) can induce the growth of albumin-producing hepatocytes in the livers of congenic Nagase’s analbuminemic rats (F344alb). Methods. F344 BMCs were cryopreserved in University of Wisconsin (UW) solution containing 10% fetal bovine serum and 12% dimethylsulfoxide, at –80℃. After thawing, 20 x 10^7 cells were infused via the portal vein into the livers of F344alb immediately after 70% hepatectomy (PH). We examined the recipient livers for albumin positive (alb+) hepatocytes and albumin mRNA and measured the serum albumin levels 4 weeks later. Results. Single and double alb+ hepatocytes were occasionally seen in the F344alb livers without the BMC transplantation. However, clusters consisting of more than three alb+ hepatocytes were seen in the livers of recipients transplanted with the cryopreserved BMCs after PH, the same as in the livers transplanted with freshly-isolated BMCs. Normal albumin mRNA was detected in the recipient livers, and the serum albumin levels were increased. Conclusion. Cryopreserved F344 BMCs can induce the growth of alb+ hepatocytes after transplantion in the F344alb liver after PH.
注記
The original publication is available at springerlink.com
