AMCoR Asahikawa Medical College
HOME
|

AMCoR:Asahikawa Medical University Collection and Research (旭川医科大学学術成果リポジトリ)は、本学で生産された電子的な知的生産物(学術雑誌論文の原稿・教材・学術資料など)を保存し、原則的に無償で発信するためのインターネット上の保管庫です。

※AMCoRに収録された学術論文のほとんどは、商業出版社や学会出版社の学術雑誌に掲載されたものですが、著作権に係わる出版社の方針により、出版社の条件に添った版を収録しています。そのため実際の誌面とはレイアウトの相違や、字句校正による文言の違いがあり得ますことをあらかじめご了承ください。


| ホーム ニュース ログイン |

Language

AMCoR検索
  
     詳細検索

インデックスツリー

詳細



閲覧数:4005
ID 8849252
アイテムタイプ Article
このアイテムを表示する
本文 1783.pdf
Type : application/pdf Download
Size : 232.4 KB
Last updated : Mar 3, 2008
Downloads : 1999

Total downloads since Feb 28, 2008 : 1999
タイトル Analysis of Phosphorylation Pathways of Antiherpesvirus Nucleosides by Varicella-Zoster Virus-Specific Enzymes
著者
古谷野, 伸 (Koyano, Shin)
Suzutani, T
Yoshida, A
Azuma, M
上位タイトル
Antimicrobial Agents and Chemotherapy Vol.40, No.4  (1996. ) ,p.920- 923
識別番号
ISSN
0066-4804
URI http://www.ncbi.nlm.nih.gov/pubmed?term=Analysis%20of%20Phosphorylation%20Pathways%20of%20Antiherpesvirus%20Nucleosides%20by%20Varicella-Zoster%20Virus-Specific%20Enzymes
抄録 The inhibitory activities of acyclovir (ACV), 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil (BV-araU), ganciclovir (GCV), 9-(2-deoxy-2-hydroxymethyl-beta-D-erythro-oxetanosyl)guanine (OXT-G), and (+)-9-[(1R,2R,3S)-2,3-bis(hydroxymethyl)Cyclobutyl]guanine (cOXT-G) on the replication of wild-type and thymidine kinase (TK)-negative strains of herpes simplex virus types 1 and 2 and varicella-zoster virus (VZV) and the wild-type strain of human cytomegalovirus were tested to clarity whether the phosphorylation of these compounds is catalyzed by viral TK or other enzymes. ACV and BV-araU had little effect on the replication of TK-negative virus strains. On the other hand, GCV, OXT-G, and cOXT-G inhibited the replication of TK-negative VZV at concentrations 10 times higher than those at which they inhibited wild-type VZV, indicating that a kinase other than TK phosphorylates GCV and OXT-G in VZV-infected cells. GCV phosphorylation activity was not detected in VZV-infected cell lysates; therefore, this activity was evaluated in COS 1 cells expressing viral TK and viral protein kinase (PK). The COS 1 cells expressing VZV TK were shown to be susceptible to all compounds tested. In contrast, VZV Pk-expressing COS 1 cells were susceptible to only GCV, OXT-G, and cOXT-G. These results suggest that VZV PK phosphorylates some nucleoside analogs, for example, GCV, OXT-G, and cOXT-G. This phosphorylation pathway may be important in the anti-VZV activities of some nucleoside analogs.
注記 Copyright © American Society for Microbiology, Antimicrobial Agents and Chemotherapy, volume 40, 920-923, 1996

publisher
言語
eng
資源タイプ text
ジャンル Journal Article
Index
/ Public
/ Public / 国外雑誌論文
関連アイテム