Muscarinic cholinoceptor-mediated activation of JNK negatively regulates intestinal secretion in mice
別タイトル
ムスカリン性アセチルコリン受容体によるJNKの活性化はマウス腸管における分泌を負に制御する
著者
Md. Tariqul, Islam
上位タイトル
Journal of pharmacological sciences
Vol.127,
No.1
(2015.
1)
,p.150-
153
識別番号
ISSN
1347-8613
DOI
10.1016/j.jphs.2014.10.001
その他
PMID：25704031
博士論文情報
学位授与番号
10107A524
学位授与年月日
2018-3-23
学位名
博士（医学）
学位授与機関
旭川医科大学
抄録
Regulation of intestinal secretion is important for body fluid homeostasis. We investigated the role of three MAP kinases (MAPKs) as negative regulators in muscarinic cholinoceptor (mAChR)-mediated intestinal secretion in mice. Electrophysiological analyses revealed that mAChR stimulation enhanced intestinal chloride secretion, which was further augmented by the inhibition of JNK but not by that of ERK or p38 with specific inhibitors SP600125, U0126 or SB203580, respectively. Immunoblot analyses in colonic mucosa showed that mAChR stimulation increased MAPKs phosphorylation that was suppressed by the specific inhibitor for each MAPK. This suggests that JNK is a major negative regulator in mAChR-induced intestinal secretion.
