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AMCoR:Asahikawa Medical University Collection and Research (旭川医科大学学術成果リポジトリ)は、本学で生産された電子的な知的生産物(学術雑誌論文の原稿・教材・学術資料など)を保存し、原則的に無償で発信するためのインターネット上の保管庫です。

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閲覧数:3269
ID 17092913
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タイトル Identification of novel keratinocyte differentiation modulating compounds by high-throughput screening
著者
本間, 大 (Honma, Masaru)
Stubbs, M
Aherne, W
Workman, P
Watt, FM
上位タイトル
Journal of biomolecular screening Vol.11, No.8  (2006. ) ,p.977- 984
識別番号
ISSN
1087-0571
DOI 10.1177/1087057106292556
URI http://www.ncbi.nlm.nih.gov/pubmed
抄録 We have attempted a high throughput-screening to find differentiation modifying compounds of keratinocyte. 11 inhibitors of known signaling pathway and approximately 4000 chemical compounds were screened using an In Cell Western system based on immunofluorescent staining. Staurosporine, a protein kinase C inhibitor and H89, a protein kinase A inhibitor, promote the expression of involucrin, a terminal differentiation marker in keratinocytes. On the other hand, U0126, a MEK1 specific inhibitor, and SAHA or SBHA, two HDAC inhibitors, prevent the expression of involucrin during calcium induced differentiation. In addition, we have found a novel compound that induces differentiation and two compounds that inhibit the differentiation of keratinocytes. One of these compounds also induced differentiation of a SCC cell line as well as apoptosis with a lower IC50 than that towards primary keratinocyte. These compounds might be useful in the future as tools to study differentiation of stratified epithelium as well as putative anti-tumor targets.
注記 ‘The final, definitive version of this paper has been published in Journal of biomolecular screening, 11/8, Dec/2006 © SAGE Publications Ltd., All rights reserved, 2006 by SAGE Publications Ltd at the Journal of biomolecular screening page: http://jbx.sagepub.com/ on SAGE Journals Online: http://online.sagepub.com/

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言語
eng
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ジャンル Journal Article
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/ Public / 国外雑誌論文
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