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AMCoR:Asahikawa Medical University Collection and Research (旭川医科大学学術成果リポジトリ)は、本学で生産された電子的な知的生産物(学術雑誌論文の原稿・教材・学術資料など)を保存し、原則的に無償で発信するためのインターネット上の保管庫です。

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閲覧数:2911
ID 15844734
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タイトル How long do parthenogenetically activated mouse oocytes maintain the ability to accept sperm nuclei as a genetic partner?
著者
立野, 裕幸 (Tateno, Hiroyuki)
Kamiguchi, Y
上位タイトル
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS Vol.22, No.2  (2005. 2) ,p.89- 93
識別番号
ISSN
1058-0468
DOI 10.1007/s10815-005-1498-0
URI http://www.springerlink.com/content/tj6275m2841kvlk2/?p=704d5ae0c6064c5083aae938666b00bc&pi=5
抄録 Purpose: Cytogenetic risk of intracytoplasmic sperm injection (ICSI) after artificial oocyte activation (post-activation ICSI) was evaluated in the mouse. Methods: Mouse zygotes were produced by ICSI into eggs at various intervals after parthenogenetic exposure to strontium (Sr) for 30 min. Male pronucleus formation and the chromosome constitution were studied. Results: Sperm nuclei injected into oocytes within 1 h after Sr exposure (from early through mid-telophase) transformed normally into male pronuclei, and the number of chromosome aberrations did not significantly increase in the resultant zygotes. When sperm nuclei were injected into eggs at intervals beyond 1 h after Sr exposure (from late telophase through the G1 pronuclear stage), the rate of male pronucleus formation was significantly reduced. The incidence of chromosome aberrations increased with time between oocyte activation and ICSI. Conclusions: ICSI into oocytes within 1 h after parthenogenetic activation produces cytogenetically competent embryos in the mouse.
注記 The original publication is available at www.springerlink.com.

author
言語
eng
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ジャンル Journal Article
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/ Public / 国外雑誌論文
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